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(A) Microscopic images of CAFs at 40 × (A) and 100 × (B) magnifications. (C) Western blot analysis of the expression of α-SMA (42 kDa), a CAF marker; β-actin (45 kDa) was used as a loading control. (D) Flow cytometry analysis of <t>FAP</t> expression in isolated CAFs. The red peak indicates cells stained with anti-FAP antibody, and the green peak represents cells stained with isotype control (anti-mouse <t>IgG</t> Ab). CAFs, cancer-associated fibroblasts; α-SMA, alpha smooth actin; FAP, <t>fibroblast</t> activation protein.
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(A) Microscopic images of CAFs at 40 × (A) and 100 × (B) magnifications. (C) Western blot analysis of the expression of α-SMA (42 kDa), a CAF marker; β-actin (45 kDa) was used as a loading control. (D) Flow cytometry analysis of <t>FAP</t> expression in isolated CAFs. The red peak indicates cells stained with anti-FAP antibody, and the green peak represents cells stained with isotype control (anti-mouse <t>IgG</t> Ab). CAFs, cancer-associated fibroblasts; α-SMA, alpha smooth actin; FAP, <t>fibroblast</t> activation protein.
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(A) Microscopic images of CAFs at 40 × (A) and 100 × (B) magnifications. (C) Western blot analysis of the expression of α-SMA (42 kDa), a CAF marker; β-actin (45 kDa) was used as a loading control. (D) Flow cytometry analysis of FAP expression in isolated CAFs. The red peak indicates cells stained with anti-FAP antibody, and the green peak represents cells stained with isotype control (anti-mouse IgG Ab). CAFs, cancer-associated fibroblasts; α-SMA, alpha smooth actin; FAP, fibroblast activation protein.

Journal: PLOS One

Article Title: The role of exosomal miRNA-125b derived from colon cancer-associated fibroblasts in skeletal muscle cachexia

doi: 10.1371/journal.pone.0342052

Figure Lengend Snippet: (A) Microscopic images of CAFs at 40 × (A) and 100 × (B) magnifications. (C) Western blot analysis of the expression of α-SMA (42 kDa), a CAF marker; β-actin (45 kDa) was used as a loading control. (D) Flow cytometry analysis of FAP expression in isolated CAFs. The red peak indicates cells stained with anti-FAP antibody, and the green peak represents cells stained with isotype control (anti-mouse IgG Ab). CAFs, cancer-associated fibroblasts; α-SMA, alpha smooth actin; FAP, fibroblast activation protein.

Article Snippet: CAFs were labeled with an anti-fibroblast activation protein (FAP) antibody (Mouse monoclonal IgG1, 427819; Novus Biologicals, Littleton, CO, USA) and subsequently stained with a fluorescein isothiocyanate-conjugated anti-mouse IgG1 secondary antibody.

Techniques: Western Blot, Expressing, Marker, Control, Flow Cytometry, Isolation, Staining, Activation Assay